PASSion: a pattern growth algorithm based pileline for splice site detection in paired-end RNA-Seq data

PASSion uses the mapped read in a pair as anchor and then uses a high resolution algorithm, pattern growth, to remap the proximal and distal fragments of the unmapped read to a local region of the reference indicated by the mate. It is capable of identifying both known and novel canonical and non-canonical junctions with SNP or sequencing error tolerance. In addition, our package can discover differential and shared splicing patterns among multiple samples.

Article

PASSion: A Pattern Growth Algorithm Based Pipeline for Splice Junction Detection in Paired-end RNA-Seq Data

Yanju Zhang; Eric-Wubbo Lameijer; Peter A.C. 't Hoen; Zemin Ning; P. Eline Slagboom; Kai Ye

Bioinformatics 2012; doi: 10.1093/bioinformatics/btr712

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Contact information

Yanju Zhang (Leiden University Medical Center, The Netherlands): y.zhang AT lumc.nl