1 | package nl.tno.metagenomics |
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2 | |
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3 | import java.util.Date; |
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4 | |
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5 | import org.codehaus.groovy.grails.commons.ConfigurationHolder |
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6 | |
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7 | class RunController { |
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8 | def fileService |
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9 | def synchronizationService |
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10 | def sampleExcelService |
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11 | def fastaService |
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12 | |
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13 | def index = { |
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14 | [runs: Run.list()] |
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15 | } |
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16 | |
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17 | def show = { |
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18 | // load run with id specified by param.id |
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19 | def run = getRun( params.id ); |
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20 | |
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21 | if (!run) { |
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22 | redirect(controller: 'study', action: 'index') |
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23 | return |
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24 | } |
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25 | |
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26 | // Make sure the newest data is available |
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27 | synchronizationService.sessionToken = session.sessionToken |
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28 | synchronizationService.user = session.user |
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29 | try { |
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30 | synchronizationService.synchronizeStudies(); |
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31 | } catch( Exception e ) { |
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32 | log.error "Exception occurred during synchronization in " + params.controller + ": " + e.getMessage() |
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33 | redirect( url: synchronizationService.gscfService.urlAuthRemote(params, session.sessionToken) ) |
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34 | } |
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35 | |
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36 | // Determine runs not used in this assay |
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37 | def otherAssays = Assay.list( sort: "name" ).findAll { !it.runs.contains( run ) && it.study.canRead( session.user ) } |
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38 | |
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39 | // Send the assay information to the view |
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40 | [run: run, allRuns: Run.list(), otherAssays: otherAssays, editable: true] |
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41 | } |
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42 | |
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43 | /** |
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44 | * Shows a form to edit the specified run in dialog mode |
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45 | */ |
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46 | def editForm = { |
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47 | // load run with id specified by param.id |
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48 | Run run = getRun( params.id ); |
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49 | |
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50 | if (!run) { |
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51 | render flash.error |
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52 | return |
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53 | } |
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54 | |
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55 | Assay assay = null |
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56 | if( params.assayId ) { |
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57 | assay = getAssay( params.assayId ) |
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58 | |
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59 | if( !assay ) { |
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60 | render flash.error; |
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61 | return |
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62 | } |
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63 | } |
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64 | |
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65 | [assay: assay, run: run] |
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66 | } |
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67 | |
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68 | def create = { |
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69 | // Retrieve the assay from the database, but don't exit with an error if no assay is found |
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70 | Assay a = getAssay(params.id); |
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71 | flash.error = ""; |
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72 | |
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73 | // Create run based on given parameters |
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74 | Run run = new Run(); |
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75 | |
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76 | run.setPropertiesFromForm( params ); |
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77 | |
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78 | if( a ) |
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79 | a.addToRuns( run ); |
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80 | |
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81 | if( !run.save() ) { |
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82 | flash.message = "Run could not be saved: " + run.getErrors(); |
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83 | } else { |
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84 | flash.message = "Run " + run.name + " has been added to the system." |
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85 | } |
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86 | |
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87 | if( a ) |
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88 | redirect( controller: "assay", action: "show", id: a.id ) |
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89 | else |
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90 | redirect( controller: 'run' ); |
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91 | } |
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92 | |
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93 | def update = { |
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94 | Run run = getRun( params.id ); |
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95 | |
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96 | if( !run ) { |
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97 | redirect(controller: 'assay', action: 'show', id: params.assayId) |
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98 | return |
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99 | } |
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100 | |
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101 | // Set properties to the run |
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102 | params.parameterFile = params.editParameterFile |
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103 | |
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104 | run.setPropertiesFromForm( params ); |
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105 | |
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106 | if( run.save() ) { |
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107 | flash.message = "Run succesfully saved"; |
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108 | } else { |
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109 | flash.error = "Run could not be saved: " + run.getErrors(); |
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110 | } |
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111 | |
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112 | Assay assay = getAssay(params.assayId); |
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113 | flash.error = ""; |
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114 | |
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115 | if( assay ) { |
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116 | redirect( controller: 'assay', action: 'show', id: assay.id) |
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117 | } else { |
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118 | redirect( controller: 'run', action: 'show', id: run.id ) |
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119 | } |
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120 | } |
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121 | |
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122 | def delete = { |
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123 | Run run = getRun( params.id ); |
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124 | |
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125 | if( !run ) { |
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126 | redirect(controller: 'assay', action: 'show', id: params.assayId) |
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127 | return |
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128 | } |
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129 | |
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130 | // Don't remove runs for which data exists |
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131 | if( run.sequenceData?.size() ) { |
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132 | flash.message = "Run could not be deleted because samples are associated with it."; |
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133 | redirect( controller: "assay", action: "show", id: params.assayId ) |
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134 | } |
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135 | |
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136 | // Check whether the user has sufficient privileges to remove the run from all assays |
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137 | def hasPrivileges = true; |
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138 | run.assay.each { |
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139 | if( !it.study.canWrite( session.user ) ) |
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140 | hasPrivileges = false |
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141 | } |
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142 | |
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143 | if( !hasPrivileges ) { |
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144 | flash.message = "Run could not be deleted because you don't have sufficient privileges to remove the run from all assays."; |
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145 | redirect( controller: "assay", action: "show", id: params.assayId ) |
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146 | } |
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147 | |
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148 | // Remove all associations |
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149 | run.assays.each { |
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150 | run.removeFromAssays( it ); |
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151 | } |
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152 | |
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153 | def name = run.name |
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154 | run.delete(); |
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155 | flash.message = "Run " + name + " has been deleted from the system." |
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156 | |
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157 | redirect( controller: "assay", action: "show", id: params.assayId ) |
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158 | } |
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159 | |
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160 | /************************************************************************** |
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161 | * |
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162 | * Methods for handling data about the samples in this run |
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163 | * |
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164 | *************************************************************************/ |
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165 | |
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166 | /** |
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167 | * Downloads an excel sheet with data about the assay samples, to enter data in excel |
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168 | */ |
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169 | def downloadTagsExcel = { |
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170 | Run run = getRun( params.id ); |
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171 | |
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172 | if( !run ) { |
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173 | redirect(controller: 'study') |
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174 | return |
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175 | } |
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176 | |
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177 | // Make it only possible to update samples writable by the user |
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178 | def assaySamples = run.assaySamples.findAll { it.assay.study.canWrite( session.user ) } |
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179 | |
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180 | def filename = "Run " + run.name + "_tags.xls" |
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181 | def wb = sampleExcelService.downloadSampleExcel( assaySamples, false ); |
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182 | |
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183 | // Make file downloadable |
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184 | log.trace( "Creation for downloading the file " + filename ) |
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185 | sampleExcelService.excelService.downloadFile( wb, filename, response ) |
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186 | } |
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187 | |
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188 | |
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189 | /** |
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190 | * Parses an uploaded excel file and shows a form to match columns |
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191 | */ |
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192 | def parseTagExcel = { |
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193 | Run run = getRun( params.id ); |
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194 | |
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195 | if( !run ) { |
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196 | redirect(controller: 'study') |
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197 | return |
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198 | } |
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199 | |
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200 | def filename = params.filename |
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201 | |
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202 | // Security check to prevent accessing files in other directories |
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203 | if( !filename || filename.contains( '..' ) ) { |
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204 | response.status = 500; |
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205 | render "Invalid filename given"; |
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206 | return; |
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207 | } |
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208 | |
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209 | // Check for existence and readability |
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210 | File file = new File( fileService.getUploadDir(), filename) |
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211 | |
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212 | if( !file.exists() || !file.canRead() ) { |
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213 | response.status = 404; |
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214 | render "The uploaded file doesn't exist or doesn't work as expected."; |
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215 | return; |
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216 | } |
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217 | |
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218 | // Save the filename in session for later use |
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219 | session.filename = filename; |
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220 | def excelData; |
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221 | try { |
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222 | excelData = sampleExcelService.parseTagsExcel( file, false ); |
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223 | } catch( Throwable e ) { // Catch a throwable here instead of an exception, since the apache poi stuff gives an Error on failure |
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224 | // Couldn't create a workbook from this file. |
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225 | response.status = 400 // Bad request |
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226 | render "Uploaded file is not a valid excel file: " + e.getMessage() |
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227 | return |
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228 | } |
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229 | session.possibleFields = excelData.possibleFields |
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230 | |
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231 | [run: run, headers: excelData.headers, exampleData: excelData.exampleData, filename: filename, possibleFields: [ "Don't import" ] + excelData.possibleFields, bestMatches: excelData.bestMatches] |
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232 | } |
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233 | |
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234 | /** |
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235 | * Updates the assay samples based on the given excel file and the column matches |
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236 | */ |
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237 | def updateTagsByExcel = { |
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238 | Run run = getRun( params.id ); |
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239 | |
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240 | if( !run ) { |
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241 | // Now delete the file, since we don't need it anymore |
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242 | _deleteUploadedFileFromSession() |
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243 | |
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244 | redirect(controller: 'study') |
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245 | return |
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246 | } |
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247 | |
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248 | if( !session.filename ) { |
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249 | // Now delete the file, since we don't need it anymore |
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250 | _deleteUploadedFileFromSession() |
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251 | |
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252 | flash.error = "No excel file found because session timed out. Please try again." |
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253 | redirect( action: 'show', id: params.id) |
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254 | return |
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255 | } |
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256 | |
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257 | // Determine the match-columns |
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258 | def matchColumns = params[ 'matches']; |
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259 | |
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260 | // Now loop through the excel sheet and update all samples with the specified data |
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261 | File file = new File( fileService.getUploadDir(), session.filename ); |
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262 | |
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263 | if( !file.exists() || !file.canRead() ) { |
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264 | flash.error = "Excel file has been removed since previous step. Please try again." |
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265 | redirect( action: 'show', id: params.id) |
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266 | return |
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267 | } |
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268 | |
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269 | // Make it only possible to update samples writable by the user |
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270 | def assaySamples = run.assaySamples.findAll { it.assay.study.canWrite( session.user ) } |
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271 | |
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272 | println "Matchcolumns: " + matchColumns |
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273 | println "Possible fields: " + session.possibleFields |
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274 | println "Assay samples: " + assaySamples.sample.name |
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275 | |
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276 | def excelData = sampleExcelService.updateTagsByExcel( matchColumns, session.possibleFields, file, assaySamples ); |
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277 | |
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278 | println excelData |
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279 | |
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280 | // Return a message to the user |
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281 | if( !excelData.success ) { |
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282 | flash.error = excelData.message |
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283 | } else if( excelData.numSuccesful == 0 ) { |
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284 | flash.error = "None of the " + excelData.failedRows.size() + " row(s) could be imported, because none of the sample names matched or no samples are writable. Have you provided the right excel file?" |
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285 | } else { |
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286 | flash.message = excelData.numSuccesful + " samples have been updated. " |
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287 | |
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288 | if( excelData.failedRows.size() > 0 ) |
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289 | flash.message += excelData.failedRows.size() + " row(s) could not be imported, because the sample names could not be found in the database or you don't have the proper permissions to change them." |
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290 | } |
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291 | |
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292 | // Now delete the file, since we don't need it anymore |
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293 | _deleteUploadedFileFromSession() |
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294 | |
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295 | redirect( action: 'show', id: params.id ) |
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296 | } |
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297 | |
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298 | |
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299 | /** |
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300 | * Update the properties of the assay samples manually |
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301 | */ |
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302 | def updateTagsManually = { |
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303 | Run run = getRun( params.id ); |
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304 | |
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305 | if( !run ) { |
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306 | redirect(controller: 'study') |
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307 | return |
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308 | } |
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309 | |
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310 | // Loop through all assay samples and set data |
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311 | def sampleParams = params.assaySample; |
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312 | |
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313 | if( sampleParams ) { |
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314 | run.assaySamples.findAll { it.assay.study.canWrite( session.user ) }.each { assaySample -> |
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315 | def assaySampleParams = sampleParams.get( assaySample.id as String ); |
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316 | if( assaySampleParams ) { |
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317 | assaySample.tagName = assaySampleParams.tagName |
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318 | assaySample.oligoNumber = assaySampleParams.oligoNumber |
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319 | assaySample.tagSequence = assaySampleParams.tagSequence |
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320 | |
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321 | assaySample.save() |
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322 | } |
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323 | } |
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324 | } |
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325 | |
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326 | flash.message = "Data about samples is saved." |
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327 | redirect( action: 'show', id: params.id ) |
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328 | } |
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329 | |
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330 | /************************************************************************** |
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331 | * |
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332 | * Methods for handling data about assays for this run |
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333 | * |
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334 | *************************************************************************/ |
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335 | |
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336 | /** |
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337 | * Adds existing samples to this run |
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338 | */ |
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339 | def addSamples = { |
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340 | Run run = getRun( params.id ); |
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341 | |
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342 | if( !run ) { |
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343 | redirect(controller: 'run', action: 'index') |
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344 | return |
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345 | } |
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346 | |
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347 | // Add checked runs to this assay |
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348 | def assaySamples = params.assaySamples |
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349 | if( assaySamples instanceof String ) { |
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350 | assaySamples = [ assaySamples ] |
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351 | } |
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352 | |
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353 | def numAdded = 0; |
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354 | assaySamples.each { assaySampleId -> |
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355 | try { |
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356 | def assaySample = AssaySample.findById( assaySampleId as Long ) |
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357 | if( assaySample.assay.study.canWrite( session.user ) ) { |
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358 | if( run.assaySamples == null || !run.assaySamples.contains( assaySample ) ) { |
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359 | run.addToAssaySamples( assaySample ); |
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360 | numAdded++; |
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361 | } |
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362 | } |
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363 | } catch( Exception e ) {} |
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364 | } |
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365 | |
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366 | flash.message = numAdded + " samples are added to this run." |
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367 | redirect( action: 'show', id: params.id) |
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368 | } |
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369 | |
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370 | /** |
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371 | * Removes sample from this run |
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372 | */ |
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373 | def removeSample = { |
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374 | Run run = getRun( params.id ); |
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375 | |
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376 | if( !run ) { |
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377 | redirect(controller: 'study') |
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378 | return |
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379 | } |
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380 | |
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381 | if( !params.assaySampleId ) { |
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382 | flash.error = "No sample id given" |
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383 | redirect(action: 'show', id: params.id) |
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384 | return |
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385 | } |
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386 | |
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387 | def assaySample |
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388 | |
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389 | try { |
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390 | assaySample = AssaySample.findById( params.assaySampleId as Long ) |
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391 | } catch( Exception e ) { |
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392 | log.error e |
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393 | flash.error = "Incorrect assaysample id given: " + params.assaySampleId |
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394 | redirect(action: 'show', id: params.id) |
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395 | return |
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396 | } |
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397 | |
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398 | if( !assaySample.assay.study.canWrite( session.user ) ) { |
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399 | flash.error = "You don't have sufficient privileges to remove the specified sample from this run." |
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400 | redirect(action: 'show', id: params.id) |
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401 | return |
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402 | } |
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403 | |
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404 | if( run.assaySamples.contains( assaySample ) ) { |
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405 | run.removeFromAssaySamples( assaySample ); |
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406 | flash.message = "The sample has been removed from this run." |
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407 | } else { |
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408 | flash.message = "The given sample was not associated with this run." |
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409 | } |
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410 | |
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411 | redirect( action: 'show', id: params.id) |
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412 | } |
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413 | |
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414 | |
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415 | /** |
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416 | * Adds existing assays to this run |
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417 | */ |
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418 | def addAssays = { |
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419 | Run run = getRun( params.id ); |
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420 | |
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421 | if( !run ) { |
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422 | redirect(controller: 'study') |
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423 | return |
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424 | } |
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425 | |
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426 | // Add checked runs to this assay |
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427 | def assays = params.assays |
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428 | if( assays instanceof String ) { |
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429 | assays = [ assays ] |
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430 | } |
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431 | |
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432 | def numAdded = 0; |
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433 | assays.each { assay_id -> |
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434 | try { |
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435 | def assay = Assay.findById( assay_id as Long ) |
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436 | if( assay.study.canWrite( session.user ) ) { |
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437 | if( run.assays == null || !run.assays.contains( assay ) ) { |
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438 | run.addToAssays( assay ); |
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439 | numAdded++; |
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440 | } |
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441 | } |
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442 | } catch( Exception e ) {} |
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443 | } |
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444 | |
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445 | flash.message = numAdded + " assays are added to this run." |
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446 | redirect( action: 'show', id: params.id) |
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447 | } |
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448 | |
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449 | /** |
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450 | * Removes assay for this run |
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451 | */ |
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452 | def removeAssay = { |
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453 | Run run = getRun( params.id ); |
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454 | |
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455 | if( !run ) { |
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456 | redirect(controller: 'study') |
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457 | return |
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458 | } |
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459 | |
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460 | if( !params.assay_id ) { |
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461 | flash.message = "No assay id given" |
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462 | redirect(action: 'show', id: params.id) |
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463 | return |
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464 | } |
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465 | |
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466 | def assay |
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467 | |
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468 | try { |
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469 | assay = Assay.findById( params.assay_id as Long ) |
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470 | } catch( Exception e ) { |
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471 | throw e |
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472 | flash.message = "Incorrect assay id given: " |
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473 | redirect(action: 'show', id: params.id) |
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474 | return |
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475 | } |
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476 | |
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477 | if( !assay.study.canWrite( session.user ) ) { |
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478 | flash.error = "You don't have sufficient privileges to remove the specified assay from this run." |
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479 | redirect(action: 'show', id: params.id) |
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480 | return |
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481 | } |
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482 | |
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483 | if( run.assays.contains( assay ) ) { |
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484 | run.removeFromAssays( assay ); |
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485 | flash.message = "The assay has been removed from this run." |
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486 | } else { |
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487 | flash.message = "The given assay was not associated with this run." |
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488 | } |
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489 | |
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490 | redirect( action: 'show', id: params.id) |
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491 | } |
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492 | |
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493 | /** |
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494 | * Exports data about one or more runs in fasta format |
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495 | */ |
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496 | def exportAsFasta = { |
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497 | def assaySamples = getAssaySamples( params ); |
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498 | |
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499 | if( assaySamples == null ) |
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500 | return; |
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501 | |
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502 | def name |
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503 | |
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504 | if( assaySamples.size() == 0 ) { |
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505 | flash.error = "No samples found for selected runs"; |
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506 | redirect( action: "list" ); |
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507 | return; |
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508 | } else if( assaySamples*.run.unique().size() == 1 ) |
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509 | name = "Run_" + assaySamples[0].run?.name?.replace( ' ', '_' ); |
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510 | else |
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511 | name = "runs"; |
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512 | |
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513 | // Export the sequences and quality scores |
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514 | response.setHeader "Content-disposition", "attachment; filename=${name}.zip" |
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515 | try { |
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516 | fastaService.export( assaySamples.unique(), response.getOutputStream(), name ); |
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517 | response.outputStream.flush(); |
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518 | } catch( Exception e ) { |
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519 | log.error( "Exception occurred during export of sequences. Probably the user has cancelled the download." ); |
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520 | } |
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521 | } |
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522 | |
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523 | /** |
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524 | * Export metadata of selected samples in excel format |
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525 | */ |
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526 | def exportMetaData = { |
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527 | def assaySamples = getAssaySamples( params ); |
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528 | def name |
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529 | |
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530 | if( assaySamples == null ) |
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531 | return; |
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532 | |
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533 | if( assaySamples.size() == 0 ) { |
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534 | flash.error = "No samples found for selected runs"; |
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535 | redirect( action: "list" ); |
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536 | return; |
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537 | } else if( assaySamples*.run.unique().size() == 1 ) { |
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538 | name = "Run_" + assaySamples[0].run?.name?.replace( ' ', '_' ); |
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539 | } else { |
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540 | name = "runs"; |
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541 | } |
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542 | |
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543 | // Export the metadata |
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544 | response.setHeader "Content-disposition", "attachment; filename=${name}.xls" |
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545 | try { |
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546 | // The export functionality needs a assaysSample-tag list, but it |
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547 | // should be empty when only exporting metadata |
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548 | def tags = []; |
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549 | assaySamples.unique().each { assaySample -> |
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550 | tags << [assaySampleId: assaySample.id, sampleName: assaySample.sample.name, assayName: assaySample.assay.name, studyName: assaySample.assay.study.name, tag: "-"] |
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551 | } |
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552 | sampleExcelService.exportExcelSampleData( assaySamples.unique(), tags, response.getOutputStream() ); |
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553 | response.outputStream.flush(); |
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554 | } catch( Exception e ) { |
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555 | log.error( "Exception occurred during export of sequences. Probably the user has cancelled the download." ); |
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556 | } |
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557 | } |
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558 | |
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559 | protected List getAssaySamples( params ) { |
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560 | def ids = params.list( 'ids' ); |
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561 | |
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562 | ids = ids.findAll { it.isLong() }.collect { Long.parseLong( it ) } |
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563 | |
---|
564 | if( !ids ) { |
---|
565 | def message = "No run ids given" |
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566 | flash.error = message |
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567 | redirect( action: "index" ); |
---|
568 | return; |
---|
569 | } |
---|
570 | |
---|
571 | def assaySamples = []; |
---|
572 | |
---|
573 | // Determine which assaySamples to export |
---|
574 | ids.each { id -> |
---|
575 | def run = Run.get( id ); |
---|
576 | if( run ) |
---|
577 | assaySamples += run.assaySamples.findAll { it.assay.study.canRead( session.user ) } |
---|
578 | } |
---|
579 | |
---|
580 | return assaySamples; |
---|
581 | } |
---|
582 | |
---|
583 | /** |
---|
584 | * Deletes an uploaded file for which the filename is given in the session. |
---|
585 | * @return |
---|
586 | */ |
---|
587 | def _deleteUploadedFileFromSession() { |
---|
588 | if( !session.filename ) |
---|
589 | return |
---|
590 | |
---|
591 | // Now delete the file, since we don't need it anymore |
---|
592 | fileService.delete( session.filename ) |
---|
593 | session.filename = '' |
---|
594 | } |
---|
595 | |
---|
596 | protected Run getRun(def runId) { |
---|
597 | // load study with id specified by param.id |
---|
598 | def run |
---|
599 | try { |
---|
600 | run = Run.get(runId as Long) |
---|
601 | } catch( Exception e ) { |
---|
602 | flash.error = "Incorrect id given: " + runId |
---|
603 | return null |
---|
604 | } |
---|
605 | |
---|
606 | if (!run) { |
---|
607 | flash.error = "No run found with id: " + runId |
---|
608 | return null |
---|
609 | } |
---|
610 | |
---|
611 | return run |
---|
612 | } |
---|
613 | |
---|
614 | protected Assay getAssay(def assayId) { |
---|
615 | // load study with id specified by param.id |
---|
616 | def assay |
---|
617 | try { |
---|
618 | assay = Assay.get(assayId as Long) |
---|
619 | } catch( Exception e ) { |
---|
620 | flash.error = "Incorrect id given: " + assayId |
---|
621 | return null |
---|
622 | } |
---|
623 | |
---|
624 | if (!assay) { |
---|
625 | flash.error = "No assay found with id: " + assayId |
---|
626 | return null |
---|
627 | } |
---|
628 | |
---|
629 | if (!assay.study.canRead( session.user ) ) { |
---|
630 | flash.error = "You don't have the right authorizaton to access assay " + assay.name |
---|
631 | return null |
---|
632 | } |
---|
633 | |
---|
634 | return assay |
---|
635 | } |
---|
636 | } |
---|