1 | package nl.tno.metagenomics |
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2 | |
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3 | import org.codehaus.groovy.grails.commons.ConfigurationHolder |
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4 | |
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5 | class AssayController { |
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6 | def synchronizationService |
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7 | def fuzzySearchService |
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8 | |
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9 | def fileService |
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10 | def excelService |
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11 | |
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12 | // Fields to be edited using excel file and manually |
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13 | def sampleNameName = "Sample name" |
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14 | def tagSequenceName = "Tag sequence" |
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15 | def oligoNumberName = "Oligo number" |
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16 | def possibleFields = [sampleNameName, tagSequenceName, oligoNumberName] |
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17 | |
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18 | def show = { |
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19 | // load study with id specified by param.id |
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20 | def assay = Assay.get(params.id as Long) |
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21 | |
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22 | if (!assay) { |
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23 | flash.message = "No assay found with id: $params.id" |
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24 | redirect('action': 'errorPage') |
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25 | return |
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26 | } |
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27 | |
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28 | // Make sure the newest data is available |
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29 | synchronizationService.sessionToken = session.sessionToken |
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30 | synchronizationService.synchronizeAssay( assay ); |
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31 | |
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32 | // Determine runs not used in this assay |
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33 | def otherRuns = Run.list( sort: "name" ).findAll { !it.assays.contains( assay ) } |
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34 | |
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35 | // Send the assay information to the view |
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36 | [assay: assay, editable: assay.study.canWrite( session.user ), otherRuns: otherRuns] |
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37 | } |
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38 | |
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39 | def showByToken = { |
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40 | // load study with token specified by param.id |
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41 | def assay = Assay.findByAssayToken(params.id as String) |
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42 | |
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43 | if (!assay) { |
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44 | // Initialize synchronizationService |
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45 | synchronizationService.sessionToken = session.sessionToken |
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46 | synchronizationService.user = session.user |
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47 | |
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48 | // If the assay is not found, synchronize studies and try again |
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49 | synchronizationService.synchronizeStudies(); |
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50 | |
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51 | assay = Assay.findByAssayToken(params.id as String) |
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52 | |
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53 | if (!assay) { |
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54 | // If the assay is not found, synchronize all studies and try again, since we might be out of sync |
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55 | synchronizationService.eager = true |
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56 | synchronizationService.synchronizeStudies(); |
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57 | |
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58 | assay = Assay.findByAssayToken(params.id as String) |
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59 | |
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60 | // If after synchronization still no assay is found, show an error |
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61 | if( !assay ) { |
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62 | flash.message = "No assay found with token: $params.id" |
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63 | redirect('action': 'errorPage') |
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64 | } |
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65 | } |
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66 | } |
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67 | |
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68 | redirect( action: "show", id: assay.id ); |
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69 | } |
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70 | |
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71 | /************************************************************************** |
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72 | * |
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73 | * Method for handling data about samples for this assay |
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74 | * |
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75 | *************************************************************************/ |
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76 | |
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77 | /** |
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78 | * Downloads an excel sheet with data about the assay samples, to enter data in excel |
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79 | */ |
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80 | def downloadTagsExcel = { |
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81 | def sheetIndex = 0; |
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82 | |
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83 | // load study with id specified by param.id |
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84 | def assay = Assay.get(params.id as Long) |
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85 | |
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86 | if (!assay) { |
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87 | flash.message = "No assay found with id: $params.id" |
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88 | redirect('action': 'errorPage') |
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89 | return |
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90 | } |
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91 | |
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92 | // Create an excel sheet |
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93 | def wb = excelService.create(); |
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94 | |
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95 | // Put the headers on the first row |
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96 | excelService.writeHeader( wb, possibleFields, sheetIndex ); |
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97 | |
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98 | // Adding the next lines |
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99 | def sortedSamples = assay.assaySamples.toList().sort { it.sample.name } |
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100 | ArrayList data = []; |
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101 | sortedSamples.each { assaySample -> |
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102 | data << [assaySample.sample.name, assaySample.tagSequence, assaySample.oligoNumber ]; |
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103 | } |
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104 | excelService.writeData( wb, data, sheetIndex, 1 ); |
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105 | |
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106 | // Auto resize columns |
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107 | excelService.autoSizeColumns( wb, sheetIndex, 0..2) |
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108 | |
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109 | // Make file downloadable |
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110 | log.trace( "Creation for downloading the file " + assay.study.name + "_" + assay.name + "_tags.xls" ) |
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111 | excelService.downloadFile( wb, assay.study.name + "_" + assay.name + "_tags.xls", response) |
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112 | } |
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113 | |
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114 | /** |
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115 | * Parses an uploaded excel file and shows a form to match columns |
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116 | */ |
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117 | def parseTagExcel = { |
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118 | def sheetIndex = 0 |
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119 | def headerRow = 0 |
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120 | def dataStartsAtRow = 1 |
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121 | def numExampleRows = 5 |
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122 | |
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123 | // load study with id specified by param.id |
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124 | def assay = Assay.get(params.id as Long) |
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125 | |
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126 | if (!assay) { |
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127 | flash.message = "No assay found with id: $params.id" |
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128 | redirect('action': 'errorPage') |
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129 | return |
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130 | } |
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131 | |
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132 | def filename = params.filename |
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133 | |
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134 | // Security check to prevent accessing files in other directories |
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135 | if( !filename || filename.contains( '..' ) ) { |
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136 | response.status = 500; |
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137 | render "Invalid filename given"; |
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138 | return; |
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139 | } |
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140 | |
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141 | // Check for existence and readability |
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142 | File file = new File( fileService.getUploadDir(), filename) |
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143 | |
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144 | if( !file.exists() || !file.canRead() ) { |
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145 | response.status = 404; |
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146 | render "The uploaded file doesn't exist or doesn't work as expected."; |
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147 | return; |
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148 | } |
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149 | |
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150 | // Save the filename in session for later use |
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151 | session.filename = filename; |
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152 | |
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153 | // Create an excel workbook instance of the file |
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154 | def workbook |
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155 | try { |
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156 | workbook = excelService.open( file ); |
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157 | } catch( Throwable e ) { // Catch a throwable here instead of an exception, since the apache poi stuff gives an Error on failure |
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158 | // Couldn't create a workbook from this file. |
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159 | response.status = 400 // Bad request |
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160 | render "Uploaded file is not a valid excel file." |
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161 | return |
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162 | } |
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163 | |
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164 | // Read headers from the first row and 5 of the first lines as example data |
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165 | def headers = excelService.readRow( workbook, sheetIndex, headerRow ); |
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166 | def exampleData = excelService.readData( workbook, sheetIndex, dataStartsAtRow, -1, numExampleRows ); // -1 means: determine number of rows yourself |
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167 | |
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168 | // Try to guess best matches between the excel file and the column names |
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169 | def bestMatches = [:] |
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170 | |
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171 | headers.eachWithIndex { header, idx -> |
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172 | // Do matching using fuzzy search. The 0.1 treshold makes sure that no match if chosen if |
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173 | // there is actually no match at all. |
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174 | bestMatches[idx] = fuzzySearchService.mostSimilar( header, possibleFields, 0.1 ); |
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175 | } |
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176 | |
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177 | [assay: assay, headers: headers, exampleData: exampleData, filename: filename, possibleFields: [ "Don't import" ] + possibleFields, bestMatches: bestMatches] |
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178 | } |
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179 | |
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180 | /** |
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181 | * Updates the assay samples based on the given excel file and the column matches |
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182 | */ |
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183 | def updateTagsByExcel = { |
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184 | def sheetIndex = 0 |
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185 | def headerRow = 0 |
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186 | def dataStartsAtRow = 1 |
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187 | |
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188 | // load study with id specified by param.id |
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189 | def assay = Assay.get(params.id as Long) |
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190 | |
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191 | if (!assay) { |
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192 | // Now delete the file, since we don't need it anymore |
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193 | _deleteUploadedFileFromSession() |
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194 | |
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195 | flash.message = "No assay found with id: $params.id" |
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196 | redirect('action': 'errorPage') |
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197 | return |
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198 | } |
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199 | |
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200 | if( !session.filename ) { |
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201 | // Now delete the file, since we don't need it anymore |
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202 | _deleteUploadedFileFromSession() |
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203 | |
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204 | flash.error = "No excel file found because session timed out. Please try again." |
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205 | redirect( action: 'show', id: params.id) |
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206 | return |
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207 | } |
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208 | |
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209 | // Determine the match-columns |
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210 | def matchColumns = params[ 'matches']; |
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211 | |
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212 | if( !matchColumns ) { |
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213 | // Now delete the file, since we don't need it anymore |
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214 | _deleteUploadedFileFromSession() |
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215 | |
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216 | flash.error = "No column matches found for excel file. Please try again." |
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217 | redirect( action: 'show', id: params.id) |
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218 | return |
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219 | } |
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220 | |
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221 | // Determine column numbers |
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222 | def columns = [:] |
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223 | def dataMatches = false; |
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224 | possibleFields.each { columnName -> |
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225 | columns[ columnName ] = matchColumns.findIndexOf { it.value == columnName } |
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226 | |
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227 | if( columnName != sampleNameName && columns[ columnName ] != -1 ) |
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228 | dataMatches = true |
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229 | } |
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230 | |
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231 | println( "Columns: " + columns) |
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232 | |
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233 | // A column to match the sample name must be present |
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234 | if( columns[ sampleNameName ] == -1 ) { |
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235 | // Now delete the file, since we don't need it anymore |
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236 | _deleteUploadedFileFromSession() |
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237 | |
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238 | flash.error = "There must be a column present in the excel file that matches the sample name. Please try again." |
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239 | redirect( action: 'show', id: params.id) |
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240 | return |
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241 | } |
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242 | |
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243 | // A column with data should also be present |
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244 | if( !dataMatches ) { |
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245 | // Now delete the file, since we don't need it anymore |
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246 | _deleteUploadedFileFromSession() |
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247 | |
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248 | flash.error = "There are no data columns present in the excel file. No samples are updated." |
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249 | redirect( action: 'show', id: params.id) |
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250 | return |
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251 | } |
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252 | |
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253 | // Now loop through the excel sheet and update all samples with the specified data |
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254 | File file = new File( fileService.getUploadDir(), session.filename ); |
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255 | if( !file.exists() || !file.canRead() ) { |
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256 | flash.error = "Excel file has been removed since previous step. Please try again." |
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257 | redirect( action: 'show', id: params.id) |
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258 | return |
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259 | } |
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260 | |
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261 | def workbook = excelService.open( file ) |
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262 | ArrayList data = excelService.readData( workbook, sheetIndex, dataStartsAtRow ) |
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263 | |
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264 | // Check whether the excel file contains any data |
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265 | if( data.size() == 0 ) { |
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266 | // Now delete the file, since we don't need it anymore |
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267 | _deleteUploadedFileFromSession() |
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268 | |
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269 | flash.error = "The excel sheet contains no data to import. Please upload another excel file." |
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270 | redirect( action: 'show', id: params.id) |
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271 | |
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272 | return |
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273 | } |
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274 | |
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275 | def numSuccesful = 0 |
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276 | def failedRows = [] |
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277 | |
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278 | // walk through all rows and fill the table with records |
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279 | def assaySamples = assay.assaySamples |
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280 | |
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281 | for( def i = 0; i < data.size(); i++ ) { |
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282 | def rowData = data[ i ]; |
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283 | |
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284 | String sampleName = rowData[ columns[ sampleNameName ] ] as String |
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285 | |
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286 | // Find assay by sample name. Since sample names are unique within an assay (enforced by GSCF), |
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287 | // this will always work. |
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288 | AssaySample assaySample = assaySamples.find { it.sample.id == Sample.findByName( sampleName )?.id }; |
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289 | |
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290 | // If no assaysample is found, add this row to the failed-row list |
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291 | if( !assaySample ) { |
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292 | failedRows << [ row: rowData, sampleName: sampleName ]; |
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293 | continue; |
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294 | } |
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295 | |
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296 | columns.each { |
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297 | if( it.value > -1 ) { |
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298 | switch( it.key ) { |
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299 | case tagSequenceName: assaySample.tagSequence = rowData[ it.value ]; break |
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300 | case oligoNumberName: assaySample.oligoNumber = rowData[ it.value ]; break |
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301 | } |
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302 | } |
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303 | } |
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304 | |
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305 | assaySample.save() |
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306 | |
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307 | numSuccesful++; |
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308 | } |
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309 | |
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310 | // Now delete the file, since we don't need it anymore |
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311 | _deleteUploadedFileFromSession() |
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312 | |
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313 | // Return a message to the user |
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314 | if( numSuccesful == 0 ) { |
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315 | flash.error = "None of the " + failedRows.size() + " row(s) could be imported, because none of the sample names matched. Have you provided the right excel file?" |
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316 | } else { |
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317 | flash.message = numSuccesful + " samples have been updated. " |
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318 | |
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319 | if( failedRows.size() > 0 ) |
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320 | flash.message += failedRows.size() + " row(s) could not be imported, because the sample names could not be found in the database." |
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321 | } |
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322 | redirect( action: 'show', id: params.id) |
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323 | } |
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324 | |
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325 | /** |
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326 | * Update the properties of the assay samples manually |
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327 | */ |
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328 | def updateTagsManually = { |
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329 | // load study with id specified by param.id |
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330 | def assay = Assay.get(params.id as Long) |
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331 | |
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332 | if (!assay) { |
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333 | flash.message = "No assay found with id: $params.id" |
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334 | redirect('action': 'errorPage') |
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335 | return |
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336 | } |
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337 | |
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338 | // Loop through all assay samples and set data |
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339 | def sampleParams = params.assaySample; |
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340 | |
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341 | if( sampleParams ) { |
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342 | assay.assaySamples.each { assaySample -> |
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343 | def assaySampleParams = sampleParams.get( assaySample.id as String ); |
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344 | if( assaySampleParams ) { |
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345 | assaySample.properties = assaySampleParams |
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346 | assaySample.save() |
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347 | } |
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348 | } |
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349 | } |
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350 | |
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351 | flash.message = "Data about samples is saved." |
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352 | redirect( action: 'show', id: params.id ) |
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353 | } |
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354 | |
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355 | /************************************************************************** |
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356 | * |
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357 | * Methods for handling data about runs for this assay |
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358 | * |
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359 | *************************************************************************/ |
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360 | |
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361 | /** |
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362 | * Adds existing runs to this assay |
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363 | */ |
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364 | def addExistingRuns = { |
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365 | // load study with id specified by param.id |
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366 | def assay = Assay.get(params.id as Long) |
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367 | |
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368 | if (!assay) { |
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369 | flash.message = "No assay found with id: $params.id" |
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370 | redirect('action': 'errorPage') |
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371 | return |
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372 | } |
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373 | |
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374 | // Add checked runs to this assay |
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375 | def runs = params.runs |
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376 | if( runs instanceof String ) { |
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377 | runs = [ runs ] |
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378 | } |
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379 | |
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380 | def numAdded = 0; |
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381 | runs.each { run_id -> |
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382 | try { |
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383 | def run = Run.findById( run_id as Long ) |
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384 | if( run.assays == null || !run.assays.contains( assay ) ) { |
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385 | run.addToAssays( assay ); |
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386 | numAdded++; |
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387 | } |
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388 | } catch( Exception e ) {} |
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389 | } |
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390 | |
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391 | flash.message = numAdded + " runs are added to this assay." |
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392 | redirect( action: 'show', id: params.id) |
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393 | } |
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394 | |
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395 | /** |
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396 | * Adds existing runs to this assay |
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397 | */ |
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398 | def removeRun = { |
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399 | // load study with id specified by param.id |
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400 | def assay = Assay.get(params.id as Long) |
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401 | |
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402 | if (!assay) { |
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403 | flash.message = "No assay found with id: $params.id" |
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404 | redirect('action': 'errorPage') |
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405 | return |
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406 | } |
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407 | |
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408 | if( !params.run_id ) { |
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409 | flash.message = "No run id given" |
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410 | redirect(action: 'show', id: params.id) |
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411 | return |
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412 | } |
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413 | |
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414 | def run |
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415 | |
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416 | try { |
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417 | run = Run.findById( params.run_id as Long ) |
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418 | } catch( Exception e ) { |
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419 | throw e |
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420 | flash.message = "Incorrect run id given: " |
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421 | redirect(action: 'show', id: params.id) |
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422 | return |
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423 | } |
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424 | |
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425 | if( assay.runs.contains( run ) ) { |
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426 | assay.removeFromRuns( run ); |
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427 | flash.message = "The run has been removed from this assay." |
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428 | } else { |
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429 | flash.message = "The given run was not associated with this assay." |
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430 | } |
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431 | |
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432 | redirect( action: 'show', id: params.id) |
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433 | } |
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434 | |
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435 | /** |
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436 | * Updates existing run |
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437 | */ |
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438 | def updateRun = { |
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439 | // load study with id specified by param.id |
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440 | def assay = Assay.get(params.id as Long) |
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441 | |
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442 | if (!assay) { |
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443 | flash.message = "No assay found with id: $params.id" |
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444 | redirect('action': 'errorPage') |
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445 | return |
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446 | } |
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447 | |
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448 | if( !params.run_id ) { |
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449 | flash.message = "No run id given" |
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450 | redirect(action: 'show', id: params.id) |
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451 | return |
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452 | } |
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453 | |
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454 | def run |
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455 | |
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456 | try { |
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457 | run = Run.findById( params.run_id as Long ) |
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458 | } catch( Exception e ) { |
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459 | throw e |
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460 | flash.message = "Incorrect run id given: " |
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461 | redirect(action: 'show', id: params.id) |
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462 | return |
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463 | } |
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464 | |
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465 | // Set properties to the run |
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466 | params.parameterFile = params.editParameterFile |
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467 | run.setPropertiesFromForm( params ); |
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468 | |
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469 | if( run.save() ) { |
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470 | flash.message = "Run succesfully saved"; |
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471 | } else { |
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472 | flash.error = "Run could not be saved: " + run.getErrors(); |
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473 | } |
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474 | |
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475 | redirect( action: 'show', id: params.id) |
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476 | } |
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477 | |
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478 | def errorPage = { |
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479 | render "An error has occured. $flash.message" |
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480 | } |
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481 | |
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482 | /** |
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483 | * Deletes an uploaded file for which the filename is given in the session. |
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484 | * @return |
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485 | */ |
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486 | def _deleteUploadedFileFromSession() { |
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487 | if( !session.filename ) |
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488 | return |
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489 | |
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490 | // Now delete the file, since we don't need it anymore |
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491 | fileService.delete( session.filename ) |
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492 | session.filename = '' |
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493 | } |
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494 | |
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495 | } |
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